Higher throughput is of the utmost importance to laboratory efficiency and profitability. Nexera MX processes twice the number of samples as conventional LCMS systems in the same amount of time.
Conventional analysis requires performing various processes such as column washing, equilibration at initial mobile phase concentrations, and the next sample injection by the autosampler during the analysis.
Therefore, demands have increased for LCMS systems that shorten the time spent for processes other than data acquisition in order to improve analytical throughput.
In an analysis of four biomarker compounds for the four major molecular species in the Cytochrome P450 family, the Nexera MX completed the analysis in only 38 seconds whereas conventional HPLC required one minute, 22 seconds.
The MX-DST incorporates a special flow line structure and instrument control system, and performs overlap control of sample injection by using two analysis systems (streams) alternately. As a result, after one system completes data acquisition, the other system starts data acquisition immediately, making it possible to use nearly the whole time of LCMS operation for data acquisition.
The SIL-40 autosampler can process the entire injection cycle time in as little as seven seconds, twice as fast as the previous model. In addition, continuous analysis can be carried out on up to 44 MTPs (using 3 PLATE CHANGERS).
Together these features dramatically increase analysis throughput.
The Nexera boasts ultra-low carryover, even on a high-sensitivity LC/MS/MS.
This reduces time spent on rinsing, resulting in a shorter overall analysis time.
With LabSolutions Insight, the MRM chromatograms to be reviewed can be displayed in a stack.
In addition, peak identification can be collectively processed via manual operation for peaks displayed. This reduces the time required for data processing, enabling more efficient data review.
LabSolutions Connect make full use of the high-throughput performance of Nexera MX. It seamlessly supports the series of operations from MRM optimization to batch queue execution, improving process efficiency.
The easily configured automatic MRM optimization function and the data browser function for checking optimization results reduce the work involved in the optimization process for multiple samples.
With LabSolutions Connect, the required information from MRM optimization results registered in the database can be accessed by loading in a text file, and then a methodfile used for analysis can be easily created. In addition, the settings for the batch file saved in a text file can be loaded, speeding up the process of batch file creation. The intuitive graphical user interface reduces the burden on the user when configuring a real-time batch analysis.
Cytochrome P450 (CYP) cocktail inhibition assay monitoring of multiple substrates is one of the major tests during early drug discovery. This section introduces thesequence of procedures for performing MRM optimization in advance for target compounds known to be substrates of CYP molecule species, creating an analysis method from the MRM transition information registered in the database, and performing real-time batch and data analysis.
1 Extract MRM transitions
2 Create method and batch files
3 Execute analysis and manage batch queue
Check the concentration values for substrates while displaying in parallel the substrates for analysis and the configured IS peaks.
Even when multiple drugs are grouped for cassette administration, it is easy to extract only the required MRM transition information from the registered database, and then create an analysis method. LabSolutions Connect is a very powerful tool for automating and heightening the efficiency of the entire sequence, from creating method files to performing analysis, and even analyzing data.
Nexera, LabSolutions Connect, LabSolutions Insight are trademarks of Shimadzu Corporation.